LOCUS       pscALPSpuro-MjACE2 (Pangolin)      10146 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses pangolin ACE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
            Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, 
            Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, 
            Kyratsous CA, Dudkina NV, Shen K, Luban J
  TITLE     Structural and Functional Analysis of the D614G SARS-CoV-2 Spike 
            Protein Variant.
  JOURNAL   Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032.
  PUBMED    32991842
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032."
COMMENT     Sequence Label: pscALPSpuro-MjACE2 (Pangolin)
FEATURES             Location/Qualifiers
     source          1..10146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..84)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        69..398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     311..330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        410..789
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        791..989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     944..964
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1007..1187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1234..1359
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1856..2089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2273..2317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2615..2732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2869..3276
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     RBS             5490..5498
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5719..5748
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6252..6851
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6252..6271)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6748..6768
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6861..7449
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6914..6934)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7332..7343)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7967..8147
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8218..8806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8298..8317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8977..9768,9769..9837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9600..9619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9838..9942)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10017..10036)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
       61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
      361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgcgttg acattgatta
      421 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      481 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      541 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      601 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      661 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      721 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      781 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      841 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      901 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      961 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     1021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1201 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1261 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1321 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1381 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1441 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1501 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1561 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1621 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1681 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     1741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaattaacaa
     2221 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2281 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2341 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2401 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2461 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2521 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2581 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2641 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2701 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2761 cagtttggtt aattaactgc agccccgata aaataaaaga ttttatttag tctccagaaa
     2821 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
     2881 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
     2941 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
     3001 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
     3061 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
     3121 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
     3181 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
     3241 ggcgcgccag tcctccgaca gactgagtcg cccgggggtc tagaagccgc caccatgagt
     3301 ggctctagtt ggcttctcct gtcactcgta gcagtgacgg cggcacaatc tacctccgat
     3361 gaagaagcta agacgtttct tgagaagttt aattcagaag cagaggaact ctcataccaa
     3421 tcctccctgg cttcatggaa ctataatacc aacatcactg atgagaacgt gcaaaagatg
     3481 aatgtagctg gggctaaatg gtccacgttt tacgaagaac agtctaagat tgccaaaaat
     3541 taccagttgc agaatattca gaacgacacg ataaaacggc aattgcaggc gttgcagctt
     3601 tcaggcagta gtgcgctgtc tgccgacaaa aatcaacgac tcaatactat cctgaatacg
     3661 atgtccacca tatattctac agggaaggta tgtaatccgg ggaatcccca ggaatgctct
     3721 ctgctggagc caggtcttga taacattatg gagtcatcta aggactacaa cgaaagactc
     3781 tgggcgtggg agggctggag aagcgaggta ggaaagcaac tccgaccact ctacgaggag
     3841 tatgtggtgc tcaaaaatga gatggctcgg gcgaaccatt atgaagacta tggtgactat
     3901 tggcgagggg attacgaggc agagggggcg aacggttata attattcacg agatcatctg
     3961 atagaggatg tagaacacat tttcacgcaa ataaagccgt tgtacgaaca cttgcatgct
     4021 tacgtcagag ctaagctgat ggataactat cctagtcaca tcagtcctac gggttgtctg
     4081 ccagcacacc ttctgggtga tatgtggggc aggttctgga ccaatctcta ccctttgact
     4141 gttccattta gacagaaacc caatattgat gtaactgatg cgatggttaa tcagacatgg
     4201 gacgctaaca ggatttttaa ggaggctgaa aagtttttcg tgtctgtagg tctgcctaag
     4261 atgacacaaa cgttctggga gaactctatg ctgacggagc cgggagacgg gcggaaagtc
     4321 gtttgccacc ccactgcctg ggatctgggg aaacacgact ttcgaatcaa aatgtgtacg
     4381 aaagtaacaa tggacgactt cctcactgcc caccacgaga tgggtcatat ccaatacgac
     4441 atggcatatg cgatgcagcc gtacctgctc cggaatgggg caaatgaagg gtttcatgag
     4501 gcggtaggcg agattatgtc actgagcgcc gcgaccccga aacatcttaa aaatatagga
     4561 ttgcttcccc ctgattttta tgaagataat gagaccgaaa tcaacttctt gcttaagcaa
     4621 gctttgacaa tagtcggaac gttgcccttt acgtacatgc tcgaaaaatg gcgctggatg
     4681 gtattttccg gccagattcc taaagaacag tggatgaaga agtggtggga aatgaaaagg
     4741 gaaatcgtag gcgtggtgga gcctgttcct catgatgaaa cttattgcga ccctgcgtca
     4801 cttttccatg tagctaatga ttattctttt atccggtatt atacgcgaac catctatcag
     4861 ttccaattcc aagaagcact ctgtcagaca gcaaagcacg agggaccgct gcataaatgc
     4921 gacatcagta atagcgccga ggcaggtcag aagctgctcc aaatgcttag cctgggcaaa
     4981 tccaagccgt ggacccttgc cctggagaga gttgtgggga ctaaaaacat ggacgtcagg
     5041 cctcttctta actacttcga gccactgttg acttggctta aagagcaaaa caaaaattcc
     5101 ttcgttggct ggaacaccga ttggagtccg tatgcggcgc aatctattaa ggttagaata
     5161 tcactcaaga gtgctttggg agaaaaagcg tatgagtgga acgactcaga aatgtatctt
     5221 tttaggagta gcgttgcgta cgcgatgcgg gaatatttta gcaaagtaaa gaagcagacg
     5281 ataccattcg aagatgagtg cgttcgcgtt tcagacctta agccgcgggt ctccttcatc
     5341 ttcttcgtta cgctccctaa aaacgtttcc gctgtcattc cgagagccga ggtagaggag
     5401 gcaattagga tttcaagatc aaggatcaac gatgcgttca gactcgacga caactctttg
     5461 gaatttcttg gaatccagcc gaccctgcaa cctccttacc agccaccagt gaccatctgg
     5521 ttgattgtct ttggggttgt catgggggta gtagtagtcg ggattgtagt tcttattttc
     5581 acagggatca gagatagaaa aaaaaaggat caggcgagat ccgaacagaa tccttatgca
     5641 agtgttgacc tgagcaaagg cgaaaataat cccggatttc aaaacgtaga cgacgtacaa
     5701 acgtctttcg gagcagctga gcagaagctg atcagcgagg aggacctgta ggaattctcg
     5761 accttgactg gcggcgcgac cttgaggcct gcgttcgcct cagttgcccc ctctgtgcaa
     5821 tggggagacg cgcctcatcg cttgacaacg gccgaagagc cgccgcgctt ccgtctcccg
     5881 cgtgcgcgcg ccatgctgcc cacccccgtt ccgcactgac cctcccccgt gccccgcgtc
     5941 ccgtactgcc gccccgcccc gagtcccatg ccgcagccac cgcgacggag cccgcaggcg
     6001 ggaacctgcc tccgcgcgtt agcgcgcacg cgcgcctcat gtgtcgtccc catcagcgcc
     6061 ggcttccgtc tataggccag atgcactgtc actctggcga agtcgcagac ccgattggcc
     6121 gggacggagg cgcgagaccg ggttgcgggc ggggccgaac gtggtataaa aggggcggga
     6181 ggccaggctc gtgccgtttt gcagacgcca ccgccgagga aaaccgtgta ctattagcca
     6241 cgcgtgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6301 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6361 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6421 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6481 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6541 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6601 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6661 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6721 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6781 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6841 ccggtgcctg agcgatcgct aatcaacctc tggattacaa aatttgtgaa agattgactg
     6901 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6961 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7021 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7081 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7141 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7201 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7261 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7321 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7381 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7441 cctccccgct taatcgcgtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7501 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     7561 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7621 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7681 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     7741 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7801 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     7861 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     7921 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     7981 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8041 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8101 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttcat
     8161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8281 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8641 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8881 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8941 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9001 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9061 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9121 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9181 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9241 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9301 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9361 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9421 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9481 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9541 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9601 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9661 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9721 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9781 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9841 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9901 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9961 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    10021 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    10081 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
    10141 atgaag
//